Anti-FOXO3 Antibody
Catalog No : IGX-59464
282.00€
201.00€
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| Description | Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PMID:10102273, PMID:16751106, PMID:21329882). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PMID:10102273, PMID:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PMID:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PMID:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation | ||
|---|---|---|---|
| Other names | Forkhead box protein O3, AF6q21 protein, FOXO3 | ||
| Cellular localization | Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus | ||
| Research area | Epigenetics & Nuclear Signaling | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | O43524 | ||
| Product name | Anti-FOXO3 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-59464 | ||
| Supplier’s Catalog No | IGX-59464 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200 | ||
| Recommended dilutions | WB: 1:500-1:5000, IHC: 1:50-1:200, IF: 1:20-1:200 | ||
| Molecular weight | Predicted band size: 72, 49 KDa Observed band size: 72-90 KDa | ||
| Conjugate | Unconjugated | ||
| Immunogen | A synthesized peptide derived from human FOXO3 | ||
| Specificity | This antibody detects total endogenous levels of FOXO3 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
|---|---|---|---|
| Clonality | Monoclonal | ||
| Isotype | Rabbit IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | ||
| Formulation | Liquid | ||
| Purification | Affinity Purified | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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