Agarose High Gel Strength

Catalog No : USB-A1030
235.64€
0.00€

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Product name Agarose High Gel Strength
Catalog No USB-A1030
Supplier’s Catalog No A1030
Supplier US Biologicals
Source antigen Gelidium
Reactivity
Cross reactivity
Applications Blotting Assays
Molecular weight
Storage RT
Other names
Grade Molecular Biology Grade
Purity
Form White, homogeeous free flowing powder
Reactivity life 12 months
Note For reserch purpose only
Description High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications. Applications: • Pulsed Field Electrophoresis • Immunoelectrophoresis • Blotting Assays (Southern, northern) • Analytical DNA Gels ≥ 1000bp • Preparative Electrophoresis • For use with all buffer systems Recommended Usage (1X TAE): Concentration Molecular Weight 0.4-2.0% 50-500kb pH (1.5%, Gel): As Reported EEO: ≤0.135 Loss on Drying: ≤7.0% Melting Point (1.5%): 86-90°C Gel Point (1.5%): 34.5-37.5°C Gel Strength (1.5%): ≥3000g/cm2 Sulfate: ≤0.10% Ash: ≤0.25% Clarity: ≤40 NTU RNase: None Detected DNase: None Detected Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. Functional and Molecular Biology Testing: To Pass Test • Comparative assay of different size DNA fragments • Southern Blot (125-23,000bp) • Background Fluorescence (TAE/TE, EtBr): • DNA Binding (TAE, EtBr) • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.