EnzyAssay™ Human TNF-α ELISA Kit
Catalog No : IGX-14863EH
384.00€
292.00€
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| Product name | EnzyAssay™ Human TNF-α ELISA Kit | ||
|---|---|---|---|
| Catalog No | IGX-14863EH | ||
| Supplier’s Catalog No | IGX-14863EH | ||
| Supplier | ImuGeX | ||
| Method | Colorimetric (450nm) | ||
| Procedure duration | 3-4 h | ||
| Application | ELISA (Sandwich) | ||
| Species reactivity | Human | ||
| Sample | Cell culture supernatants, Serum, Plasma, other biological fluids | ||
| Detection limit | 4 pg/mL | ||
| Detection range | 7.8 - 500 pg/mL | ||
| Storage | 2-8°C & -20°C | ||
|---|---|---|---|
| Other names | TNF alpha, TNFα, TNF, DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein, TNF superfamily, member 2, TNF, macrophage-derived, TNF, monocyte-derived, cachectin, tumor necrosis factor alpha, TNFSF7 | ||
| Reactivity life | 6 months when stored properly at recommended temperature. | ||
| Note | This product is for research purpose only, not for diagnostics or therapeutics. | ||
| Description | TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes. EnzyAssay™ Human TNF-α ELISA Kit has high sensitivity and excellent specificity for detection of Human TNF-α. No significant cross-reactivity or interference between Human TNF-α and analogues was observed. | ||
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