Anti-PMS2 antibody
Catalog No : FT-FNab06578
294.00€
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| Description | Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha(MSH2-MSH6) or MutS beta(MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha(MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. | ||
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| Research area | Epigenetics, Metabolism, Cancer, Immunology, Developmental biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | P54278 | ||
| Product name | Anti-PMS2 antibody | ||
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| Catalog No | FT-FNab06578 | ||
| Supplier’s Catalog No | FNab06578 | ||
| Supplier | Fine Test (Wuhan Fine Biotech) | ||
| Host species | Rabbit | ||
| Species reactivity | Human, Mouse, Rat | ||
| Applications | ELISA, WB, IHC | ||
| Recommended dilutions | WB: 1:500-1:2000, IHC: 1:50-1:500 | ||
| Molecular weight | 100 kDa | ||
| Conjugate | Unconjugated | ||
| Immunogen | PMS2 postmeiotic segregation increased 2(S. cerevisiae) | ||
| Specificity | N/A | ||
| Storage | Store at -20°C. Avoid repeated freeze and thaw cycles. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3 | ||
| Formulation | Liquid | ||
| Purification | Immunogen affinity purified, ≥95% as determined by SDS-PAGE | ||
| Handling | This product contains NaN3 as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions. | ||
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