Anti-SARS-CoV-2 Spike RBD Antibody (Nanobody) Monoclonal
Catalog No : IGX-29576
475.00€
314.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Description | This SARS-CoV-2 S1-RBD (Spike Glycoprotein S1 receptor-binding domain) antibody is a recombinant monoclonal antibody (also a Nanobody) generated through the expression of a DNA sequence inserting a human IgG1 Fc domain at the C-terminus, in human embryonic kidney 293 cells (HEK293). The DNA sequence encodes the SARS-CoV-2 spike RBD. The antibody is purified by protein G in vitro. It has been validated with high reactivity towards SARS-CoV-2 S1-RBD by a functional ELISA and good sensitivity for human SARS-CoV-2 spike glycoprotein (S protein) via the Colloidal Gold Immunochromatography Assay (GICA). It is also validated in Neutralizing and LSPR. In neu assay, the binding signal of the SARS-CoV-2 S1 RBD antibody was inhibited by ACE2 protein-HRP conjugated inhibitor, with a 0.1074 μg/mL IC50. In LSPR assay, the SARS-CoV-2 S1 RBD antibody showed a high affinity with SARS-CoV-2 Spike protein RBD (affinity constant: 28.2nM). Specifically binding and recognizing the RBD of the SARS-CoV-2 spike glycoprotein, the SARS-CoV-2 S1 RBD antibody can react with samples infected with human coronavirus SARS-CoV-2. But it does not respond to MERS or SARS-CoV spike protein. Akin to other nanobodies, this recombinant nanobody is small and stable, which allows for its reaching to hidden epitopes such as crevices of target proteins. | ||
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| Other names | Coronavirus spike Antibody, CoV spike Antibody, nCoV RBD Antibody, ncov S1 Antibody, nCoV spike Antibody, Novel coronavirus RBD Antibody, Novel coronavirus S1 Antibody, Novel coronavirus spike Antibody, RBD Antibody, S1 Antibody, Spike RBD Antibody, E2 Antibody, E2 glycoprotein Antibody, Human coronavirus spike glycoprotein Antibody, S Antibody, SARS-CoV-2 S1 RBD Antibody, S glycoprotein Antibody, Spike glycoprotein Antibody | ||
| Cellular localization | Host cell membrane | ||
| Research area | Immunology, Cardiovascular, Neuroscience, Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | P0DTC2 | ||
| Product name | Anti-SARS-CoV-2 Spike RBD Antibody (Nanobody) Monoclonal | ||
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| Catalog No | IGX-29576 | ||
| Supplier’s Catalog No | IGX-29576 | ||
| Supplier | ImuGeX | ||
| Host species | Mouse | ||
| Species reactivity | Human Novel Coronavirus | ||
| Applications | ELISA, GICA, Neutralising | ||
| Recommended dilutions | ELISA: 1:10000-1:100000, GIGA: 1:10000-1:40000, Neutralising: 1:100-1:10000 | ||
| Molecular weight | 141 KDa | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Novel Coronavirus Spike glycoprotein (AA: 319-541) | ||
| Specificity | This antibody binds to SARS-CoV-2 spike protein. | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Monoclonal | ||
| Isotype | VHH fusion with human IgG1 Fc | ||
| Clone ID | A1 | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-Buffered Saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | >95%, Protein G purified | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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