Purified Anti-Mouse CD45.2 Antibody (clone 104)
Catalog No : IGX-1010
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| Description | CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. CD45.2 (clone 104) antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells. | ||
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| Other names | Ly5.2, B220, CD45R, L-CA, loc, Ly-5, Lyt-4, Ptprc, T200, B220, c polypeptide, CD45, CD45 antibody, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, Ly-5, LY5, Lymphocyte antigen 5, lymphocyte common antigen, protein tyrosine phosphatase, PTPRC, receptor type, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen | ||
| Cellular localization | Plasma membrane | ||
| Research area | Immunology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | P06800 | ||
| Product name | Purified Anti-Mouse CD45.2 Antibody (clone 104) | ||
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| Catalog No | IGX-1010 | ||
| Supplier’s Catalog No | IGX-1010 | ||
| Supplier | ImuGeX | ||
| Host species | Mouse | ||
| Species reactivity | Mouse | ||
| Applications | Flow cyto, IHC (Frozen), ICC, IF, IP | ||
| Recommended dilutions | Flow cyto: 0.5 µg/10^5-8 cells. ICC/IF, ICH, IP: Use at an assay dependent concentration. | ||
| Molecular weight | 145 KDa | ||
| Conjugate | Unconjugated | ||
| Immunogen | Tissue or cells corresponding to mouse CD45.10 | ||
| Specificity | The antibody has been demonstrated to be specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells. | ||
| Storage | Store at 4°C. Do not freeze. | ||
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| Clonality | Monoclonal | ||
| Isotype | IgG2a, kappa | ||
| Clone ID | 104 | ||
| Concentration | 0.5 mg/mL | ||
| Storage buffer | 10 mM NaH2PO4 (pH7.2), 150 mM NaCl, 0.09% NaN3. | ||
| Formulation | Liquid | ||
| Purification | This antibody was purified from tissue culture supernatant via affinity chromatography, >90% pure as determined by SDS-PAGE. | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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