Anti-PERK Antibody

Catalog No : IGX-7104
297.00€
351.00€

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Description Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Other names EIF2AK3, Eukaryotic translation initiation factor 2-alpha kinase 3, EC 2.7.11.1, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK
Cellular localization Endoplasmic Reticulum Membrane, Single-Pass Type I Membrane
Research area Immunology
Note This product is for research purpose only, not for use in diagnostics or as therapeutics.
Uniprot ID Q9NZJ5
Product name Anti-PERK Antibody
Catalog No IGX-7104
Supplier’s Catalog No IGX-7104
Supplier ImuGeX
Host species Rabbit
Species reactivity Human, Mouse, Rat
Applications WB, IHC, IF, ICC, ELISA
Recommended dilutions WB: 1:500-1:2000, ICH: 1:50-1:200, IF/ICC: 1:100-1:500, ELISA: 1:20000-1:40000 IHC: 1:20-1:200
Molecular weight Predicted band size: 125 KDa, Observed band size: 125~140 KDa
Conjugate Unconjugated
Immunogen A synthesized peptide derived from Human EIF2AK3, corresponding to amino acid residues D965-E994.
Specificity PERK antibody detects endogenous levels of total PERK.
Storage Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity.
Clonality Polyclonal
Isotype IgG
Clone ID N/A
Concentration 1 mg/mL
Storage buffer Phosphate buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Formulation Liquid
Purification Antigen Affinity Purified
Handling This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product.
Reactivity life 12 months when stored properly according to instructions (provided antibody remains sterile).