Anti-phospho-PERK (Thr982) Antibody
Catalog No : IGX-7106
297.00€
351.00€
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| Description | Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. | ||
|---|---|---|---|
| Other names | EIF2AK3, Eukaryotic translation initiation factor 2-alpha kinase 3, EC 2.7.11.1, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3, PEK, PERK | ||
| Cellular localization | Endoplasmic Reticulum Membrane, Single-Pass Type I Membrane | ||
| Research area | Immunology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q9NZJ5 | ||
| Product name | Anti-phospho-PERK (Thr982) Antibody | ||
|---|---|---|---|
| Catalog No | IGX-7106 | ||
| Supplier’s Catalog No | IGX-7106 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human, Mouse, Rat | ||
| Applications | WB, IHC, IF, ICC, ELISA | ||
| Recommended dilutions | WB: 1:1000-3000, ICH: 1:50-1:200, IF/ICC: 1:100-500, ELISA: 1:20000-1:40000 | ||
| Molecular weight | Predicted band size: 125 KDa, Observed band size: 125~140 KDa | ||
| Conjugate | Unconjugated | ||
| Immunogen | A synthesized peptide derived from Human EIF2AK3 around phosphorylation site Thr982, corresponding to amino acid residues T970-L989. | ||
| Specificity | Phospho-PERK (Thr982) antibody detects endogenous levels of PERK only when phosphorylated at Thr982. | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
|---|---|---|---|
| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate buffered saline (pH 7.4) containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. | ||
| Formulation | Liquid | ||
| Purification | Antigen Affinity Purified | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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