Agarose DNA Fragments 50-2000bp (3:1 Blend)
Catalog No : USB-A1012
240.24€
0.00€
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| Product name | Agarose DNA Fragments 50-2000bp (3:1 Blend) | ||
|---|---|---|---|
| Catalog No | USB-A1012 | ||
| Supplier’s Catalog No | A1012 | ||
| Supplier | US Biologicals | ||
| Source antigen | Gelidium | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | Blotting Assays | ||
| Molecular weight | |||
| Storage | RT | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | White, homogeeous free flowing powder | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Agarose for DNA Fragments 50-2000bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. Manufactured specifically for primer size fragments in the range of 50-2000 base pairs and exhibits exceptionally high transparency even at high concentrations (5%). Specially purified to be free of background contamination that could interfere with restriction digests or ligations and selected for use in PCR product analysis. Applications: • Analytical DNA Gels ≤ 2000bp • PCR products • Restriction Enzyme digestion products • Capillary Electrophoresis • Mutation Analysis • For use with all buffer systems Recommended Usage (1X TAE): Concentration Molecular Weight 1-4% 50-2000bp 1% 500-2000bp 2% 250-750bp 3% 125-500bp 4% 50-250bp Note: A 3% agarose gel is equivalent to 8% polyacrylamide Source: Gelidium Form: White, homogeeous free flowing powder EEO: ≤0.12 Absorbance (450nm,1.5%): As reported Melting Temperature: ≤93°C Gelling Temperature: ≤40.5°C Gel Strength (1.5%): ≥2000g/cm2 Gel Strength (4%): ≥4250g/cm2 pH in Solution (1.5%,H2O): 5.0-7.0 Loss on Drying: ≤7.0% Sulfate: 0.1% Ash: ≤0.35% RNase: None Detected DNase: None Detected DNA Binding: To Pass Test Background Fluorescence (EtBr): To Pass Test Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. Functional and Molecular Biology Testing: To Pass Test • Comparative assay of different size DNA fragments • Southern Blot (125-23,000bp) • Background Fluorescence (TAE/TE, EtBr): • DNA Binding (TAE, EtBr) • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. | ||
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