DNA, Salmon, (Protein A Agarose)
Catalog No : USB-D3956
612.66€
0.00€
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| Product name | DNA, Salmon, (Protein A Agarose) | ||
|---|---|---|---|
| Catalog No | USB-D3956 | ||
| Supplier’s Catalog No | D3956 | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | Blotting Assays | ||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | |||
| Form | Supplied as a 50% slurry of agarose beads in 10mM Tris-HCl, 1mM EDTA, pH8, 0.05% sodium azide, 1mg/ml BSA, 3mg/ml Protein A. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Recombinant Protein A covalently bound to agarose by alkylamine linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein A agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIPs assays). Applications: Suitable for use in Chromatin Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Quality Control: Immunoprecipitation: 80ul of gel slurry pre-cleared a chromatin solution and subsequently 60ul immunoprecipitated DNA cross-linked to acetylated histones using H5110-13A1: Histone H3, acetylated (Lys9, Lys14). Testing was performed in conjunction with H5110-21: Histone H4, acetylated, Immunoprecipitation Assay Kit, BioAssay™. This agarose was also tested with H5110-13Y: Histone H3, acetylated, Immunoprecipitation Assay Kit, BioAssay™. Storage and Stability: Stable for 1 year at 4ºC. Do not freeze. | ||
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