PAK 1 PBD (Rac/cdc42) Assay Reagent, Recombinant, Human (Agarose)
Catalog No : USB-P2488-05
424.15€
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| Product name | PAK 1 PBD (Rac/cdc42) Assay Reagent, Recombinant, Human (Agarose) | ||
|---|---|---|---|
| Catalog No | USB-P2488-05 | ||
| Supplier’s Catalog No | P2488-05 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates. Species Crossreactivity: Predicted to interact with all mammalian Rac and cdc42. | ||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~90% (SDS-PAGE and Coomassie blue staining) | ||
| Form | Supplied as a liquid suspension, bound to glutathione agarose beads; provided as a 50% slurry in PBS, pH 7.4, 50% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | GST-tagged fusion protein to residues 67-150 of human PAK-1, expressed in E. coli, bound non-covalently to glutathione-agarose. Applications: Suitable for use in Affinity Precipitation and Immmunoblot. Other applications have not been tested. Recommended Dilutions: Affinity Precipitation: 10ug precipitated Rec-GTP and cdc42-GTP fromn HeLa cell lysates that were loaded in vitro with GTP-gammaS and GDP. Immunoblot: 1ug/ml Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe | ||
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