Raf 1 RBD, Recombinant, Human (Agarose)
Catalog No : USB-R0495-15
771.29€
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| Product name | Raf 1 RBD, Recombinant, Human (Agarose) | ||
|---|---|---|---|
| Catalog No | USB-R0495-15 | ||
| Supplier’s Catalog No | R0495-15 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified (~80%). | ||
| Form | Provided as a 50% agarose gel slurry in PBS, 50% glycerol. Liquid suspension. Bound to glutathione agarose. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. Human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. Applications: Suitable for use in Ras affinity precipitation. Other applications not tested. Recommended Dilution: Ras affinity precipitation: 5-10ug. Precipitates Ras-GTP from HeLa lysates that were loaded in vitro with GTPgS and EGF-stimulated HeLa cells that were lysed in a magnesium containing lysis buffer. Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 1 month. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Also Available Ras Products: Enzymes: R0495-15: Raf 1 RBD, Recombinant, Human (Agarose) R1198-20: Ras, GST (Agarose) Antibodies: R1198: Ras, p21, N,H,K Mab Mo xHu Kits: R1198-07: Ras Activation Assay Kit, BioAssay™ Ha-Ras Products: Antibodies: R1198-34: Ras, H (Harvey Murine Sarcoma Virus Oncogene, Ha-Ras) Mab Rb xHu RasGAP Products: Antibodies: R1198-11: Ras, GAP (Ras GTPase Activating Protein, GAP) Mab Mo xHu R1198-12: Ras, GAP (Ras GTPase Activating Protein, GAP) Pab Rb xHu | ||
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