Imugex

Shipping cost plus VAT not included , delivery in 7-14 business days

Categories: Biochemicals / Biochemicals

Product name			Ral BP1, Recombinant, Rat (Ral Assay Reagent) (Agarose)
Catalog No			USB-R1035-15
Supplier’s Catalog No			R1035-15
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Highly Purified
Purity			Highly Purified (~95%)
Form			Supplied as a liquid suspension, 50% agarose slurry in 50mM Tris, pH 7.5, 200mM sodium chloride, 1% NP40, 1mM PMSF, 1mM aprotinin prior to the addition of glycerol to 50%.
Reactivity life			6 months
Note			For reserch purpose only
Description			Source: GST-tagged fusion protein corresponding to residues 397-519 of rat Ral binding protein, expressed in E. coli Applications: Afﬁnity Precipitation: 10-15ul (5-7.5ug) of a 50% slurry precipitated RalAGTP and RalB-GTP from 1mg of EGF-stimulated Cos-7 cells which had been lysed in a magnesium containing lysis buffer. Note: Due to spontaneous deactivation of RalA and RalB, use lysates that have been stored at-70°C for less than 1 month. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.