Ovalbumin (Agarose)
Catalog No : USB-O8075-02
587.37€
0.00€
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| Product name | Ovalbumin (Agarose) | ||
|---|---|---|---|
| Catalog No | USB-O8075-02 | ||
| Supplier’s Catalog No | O8075-02 | ||
| Supplier | US Biologicals | ||
| Source antigen | Chicken egg ovalbumin | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 43000 | ||
| Storage | 4°C Do not freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | The affinity matrix is supplied in PBS pH 7.4 containing 0.05% azide. The column has a binding capacity of ~2-10mg anti-Ovalbumin per ml of beads. Typically, this may corresponds to 5-10ml antiserum containing antibodies to Ovalbumin. The anti-Ovalbumin IgG may vary and therefore, binding capacity of the Ovalbumin-agarose must be evaluated for each batch of antiserum. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Many antibodies are made to small peptides or haptens that are injected as Ovalbumin-conjugates. Antibodies are produced against the carrier protein (Ovalbumin) and the coupled peptide or hapten. Anti-Ovalbumin antibodies may interfere with some analyses. The Ovalbumin-agarose column can be used to remove the anti- Ovalbumin antibodies using standard antibody purification techniques. Applications: Suitable for use in removing anti-ovalbumin antibodies in antisera. Other applications not tested. Usage: We recommend processing approximately 1-5ml antiserum per 1ml of the beads or it can be scaled up accordingly. Load antiserum diluted 1:5 in PBS to adsorb anti-Ovalbumin at room temperature. Collect unbound fraction containing Ovalbumin-depleted antiserum. It may be necessary to repeat this adsorption if the sample contains high concentrations of ant-Ovalbumin. Regeneration: The column can be regenerated by passing 3ml of 0.1M Glycine buffer, pH 2.5, and then immediately washing with with 10-20 volumes of PBS, pH 7.4. Store column in PBS containing 0.05% azide at 4°C. DO NOT FREEZE the beads at any stage. Storage and Stability: Stored at 4°C. Do not freeze. | ||
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