Imugex

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Categories: Biochemicals / Biochemicals

Product name			Protein L, Recombinant, Peptostreptococcus Magnus (Agarose)
Catalog No			USB-P9106E
Supplier’s Catalog No			P9106E
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			4°C Do Not Freeze
Other names
Grade			Purified
Purity			Purified.
Form			Supplied as a 50% slurry in 0.01% thimerosal/H2O.
Reactivity life			12 months
Note			For reserch purpose only
Description			Protein L Agarose is prepared by covalently coupling recombinant Protein L (contains five Ig light chain binding domains, catalog # P9106D) to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity. SOURCE: E. coli Endotoxin level: ≤0.1EU/mg Applications: Purification of monoclonal and polyclonal antibodies and Immunoprecipitation. Other applications not tested. Binding Capacity: IgG binding is greater than 10mg of human IgG per 1ml of wet gel. High flow rate. Low falling off of rProtein A/G. pH stability 2-10. Specificity: Protein L binds to all IgG subclasses from human, mouse and rat species. It also binds to human, mouse, and rat IgM, IgA, IgE, and IgD, as well as Fab and K light chains. Protein L is also superior for binding to chicken, Hamster and pig IgG. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.