Imugex

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Categories: Biochemicals / Biochemicals

Storage			4°C Do Not Freeze
Other names
Grade
Purity
Form			Supplied as a 50% slurry in 0.01% Thimerosal / H2O; > 3mg (10umol) glutathione per ml Sepharose beads.
Reactivity life			12 months
Note			For reserch purpose only
Description			The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the over expression of foreign genes in E. coli. The expressed fusion protein with a GST tail can be easily purified by affinity chromatography on Glutathione-Sepharose beads from the bacterial lysate. Glutathione-Sepharose is designed for the purpose of purification of such GST fusion proteins or any other kinds of glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10. Applications: Suitable for use for purification of GST-fusion proteins or other glutathione- binding proteins. Bead Structure: 4% cross-linked spherical agarose Mean particle size: 90um (45-165um) Ligand: Glutathione pH stability: 2-10 Chemical stability: 1M NaOH (1wk, 20°C) 0.01M NaOH , pH 12 0.01M HCl, pH 2 4M sodium chloride 8M urea 6M guanidine hydrochloride Storage buffer: 0.01% Thimersol / ddH2O Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.