CpG-DNA, Mouse, BioGenomics™
Catalog No : USB-C7905-85
663.24€
0.00€
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| Product name | CpG-DNA, Mouse, BioGenomics™ | ||
|---|---|---|---|
| Catalog No | USB-C7905-85 | ||
| Supplier’s Catalog No | C7905-85 | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified | ||
| Form | Supplied as a lyophilized powder. Reconstitute with dH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Bacterial DNA is known to induce acute inflammatory responses. Bacterial DNA acts as a pathogen-associated molecular pattern by virtue of a 20-fold greater frequency of unmethylated CG dinucleotides found in microbial DNA versus vertebrate DNA. Cellular activation by deoxy-cytidylate-phosphate-deoxy- guanylate (CpG)-DNA occurs via the Toll/IL-1R signal pathway. TLR9 appears to be a major component of the CpG-DNA receptor, acting by direct binding to CpG-DNA. The synthetic oligodeoxynucleotides (ODN) contain the proper CpG-DNA motif mimicing the immunostimulatory effects of bacterial DNA. The human optimal CpG motif GTCGTT differs from the optimal mouse CpG motif GACGTT. Non CpG-DNA has been shown to compete with CpG-DNA in in vitro stimulation experiments. Source: This mouse CpG is a 20-mer ODN has the following sequence: 5'-tccatgacgttcctgatgct-3'. Regular letters represent phosphorothiorate linkage. Applications: Suitable for use for biological assays in vitro to activate murine cells. Other applications not tested. Recommended Dilutions: In vitro Stimulation: 0.01-10uM Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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