Baculovirus Expression Vector pAc-l-Fc

Catalog No : USB-E9073-56
713.81€
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Product name Baculovirus Expression Vector pAc-l-Fc
Catalog No USB-E9073-56
Supplier’s Catalog No E9073-56
Supplier US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Purified
Purity Purified using a Plasmid Purification Kit.
Form Supplied as a liquid in TE buffer.
Reactivity life 12 months
Note For reserch purpose only
Description Baculovirus cassette vector pAc-l-Fc for the expression of human, humanized or chimeric IgG(lambda) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fab antibody gene fragments. Vector Size: 6701bp Resistance: Vector contains an ampicillin resistance marker gene Applications: Suitable for use in cloning of heavy and light chain variable domain genes of scFv or Fab antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries. Cloning sites: XhoI and SpeI for heavy chain Fab fd genes SacI and EcoRV for light chain Fab(l) genes. Expression of immunoglobulin heavy and light chains in insect cells and secretion of assembled IgG(l) antibodies into the supernatant. Storage and Stability: For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.