HIV-1 pol Integrase, Recombinant (Human Immunodeficiency Virus-1)
Catalog No : USB-H6003-15C
427.60€
0.00€
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| Product name | HIV-1 pol Integrase, Recombinant (Human Immunodeficiency Virus-1) | ||
|---|---|---|---|
| Catalog No | USB-H6003-15C | ||
| Supplier’s Catalog No | H6003-15C | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% by HPLC and SDS-PAGE | ||
| Form | Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 1.5M urea, 0.2% Triton-X, 50% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% by HPLC and SDS-PAGE | ||
| Description | Integrase is an enzyme produced by the HIV which enables its genetic material to be integrated into the DNA of the infected cell and is a key component in the pre-integration complex. HIV integrase contains 3 domains, an N-terminal HH-CC zinc fingerdomainwhich is partially responsible for multimerization, a central catalytic domain and a C-terminal domain. Both Central catalytic domain and C-terminal domains have been shown to bind both viral and cellular DNA. No crystal structure data exists with Integrase bound to its DNA substrates. HIV-1 integrase functions as a dimeror a tetramer. Additionally, several host cellular proteins interact with integrase and may facilitate the integration process. Description: The E.coli derived 26kD recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the pol protein (intergrase) and fused with six histidines at the N-terminus. Applications: Suitable for use as an antigen in ELISA and Western Blot. It is an excellent antigen for early detection of HIV seroconvertors with minimal specificity problems. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specificity: Immunoreactive with all sera of HIV-I infected individuals. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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