Rubella, gpE1, Mosaic, Recombinant (aa157-176, 374-390, 213-239) (German Measles)

Catalog No : USB-R9700-10
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Product name Rubella, gpE1, Mosaic, Recombinant (aa157-176, 374-390, 213-239) (German Measles)
Catalog No USB-R9700-10
Supplier’s Catalog No R9700-10
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form Supplied as a liquid in 20mM imidazole, 8M urea, 0.3M sodium chloride.
Reactivity life 6 months
Note For reserch purpose only
Purity ≥ 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Description Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase. The protein contains glycoprotein E1 immunodominant fragments, amino acids: (157-176), (374-390), (213-239). Specificity: Immunoreactive with sera of Rubella Virus infected individuals. Applications: Antigen in ELISA and Western Blots, excellent antigen for detection of Rubella Virus with minimal specificity problems. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.