Hepatitis A Virus VP4-VP2, aa55-164, Recombinant (HAV)

Catalog No : USB-H1900-49A
456.33€
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Product name Hepatitis A Virus VP4-VP2, aa55-164, Recombinant (HAV)
Catalog No USB-H1900-49A
Supplier’s Catalog No H1900-49A
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 44
Storage -20°C
Other names
Grade Highly Purified
Purity Purified by proprietary chromatographic techniques. ≥90% pure as determined by 10% PAGE (coomassie staining).
Form Supplied as a liquid in 10mM CBB, pH 9.6, 0.1% SDS, 50% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified by proprietary chromatographic techniques. ≥90% pure as determined by 10% PAGE (coomassie staining).
Description HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions. Recombinant corresponding to aa55-164 of HAV structural proteins VP2-VP4 immunodominant regions expressed in E. coli. Immunoreactive with sera of HAV-infected individuals. Applications: Suitable for use in ELISA and Western Blot. Excellent antigen for detection of HAV with minimal specificity problems. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.