Hepatitis C Virus NS3, aa1400-1643, Recombinant (HCV)
Catalog No : USB-H1920-23N
456.33€
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| Product name | Hepatitis C Virus NS3, aa1400-1643, Recombinant (HCV) | ||
|---|---|---|---|
| Catalog No | USB-H1920-23N | ||
| Supplier’s Catalog No | H1920-23N | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 22 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Purified by proprietary chromatographic techniques. ≥ 95% pure as determined by 10% PAGE (coomassie staining). | ||
| Form | Supplied as a liquid in 25mM Tris-HCl, 1mM EDTA, 1.5M urea and 50% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified by proprietary chromatographic techniques. ≥ 95% pure as determined by 10% PAGE (coomassie staining). | ||
| Description | The nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region. Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around aa1250/1251), A (within aa1250-1334), A/B (around aa1323 and 1334), and B/C (around aa1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis E. coli derived recombinant from HCV. The protein contains the HCV NS3 (c33c) immunodominant fragment, linear sequence of amino acids: 1400-1643. Applications: Antigen in ELISA and Western Blot, excellent antigen for detection of HCV with minimal specificity problems. Immunoreactive with sera of HCV-infected individuals, including sera with antibodies to NS3 secondary domains. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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