Hepatitis C Virus NS3, Subtype 1b, aa1359-1459, Recombinant (HCV)
Catalog No : USB-H1920-23P
456.33€
0.00€
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| Product name | Hepatitis C Virus NS3, Subtype 1b, aa1359-1459, Recombinant (HCV) | ||
|---|---|---|---|
| Catalog No | USB-H1920-23P | ||
| Supplier’s Catalog No | H1920-23P | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Purified by proprietary chromatographic techniques. ≥ 95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. | ||
| Form | Supplied as a liquid in 25mM Tris-HCl, 1mM EDTA, 1.5M urea and 50% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified by proprietary chromatographic techniques. ≥ 95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. | ||
| Description | The nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region. Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around aa1250/ 1251), A (within aa1250-1334), A/B (around aa1323 and 1334), and B/C (around aa1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis E. coli derived recombinant from HCV subtype 1b. The protein contains the full-length HCV NS3 (c33c) immunodominant region, aa1359-1459. Applications: Recombinant HCV NS3 Antigen may be used in ELISA and Western Blots, excellent for detection of HCV with minimal specificity problems. Immunoreactive with sera of HCV-infected individuals. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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