Oligo, Clockwise Sequencing Primer, pBR322 (BamH I), (20-mer), [5’-CAC TAT CGA CTA CGC GAT CA-3’]

Catalog No : USB-O6020-27
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Product name Oligo, Clockwise Sequencing Primer, pBR322 (BamH I), (20-mer), [5’-CAC TAT CGA CTA CGC GAT CA-3’]
Catalog No USB-O6020-27
Supplier’s Catalog No O6020-27
Supplier US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Molecular Biology Grade
Purity Purified by HPLC. Quality Control: MALDI-TOF MS
Form Supplied as a lyophilized powder. Reconstitute with sterile dH2O, TE or Tris buffer, pH 7.0.
Reactivity life 12 months
Note For reserch purpose only
Description Single-stranded oligonucleotide, chemically synthesized, suitable for DNA applications. This sequencing primer is used to sequence DNA that has been cloned into pBR322. Special Labels: Biotin, Fluorescein, and Phosphate modifications can be included at the 5' or 3' site. For special labels add -B, -F, -P and -5, -3 to the catalog number. Sequence: 5' CAC TAT CGA CTA CGC GAT CA 3' Molecular Weight: 6046g/mol GC Content: 50% Tm: 57.3°C Synthesis Scale: 50nmole Synthesis scale refers to the amount of the 3'-terminus base that the multi-step synthesis starts with. Final yield is indicated on the lot-specific certificate of analysis. Minimum Guaranteed Yield: 1.0 OD (A260nm) unit per mL reconstituted product 1 OD unit corresponds to ~33ug ssDNA Storage & Stability: Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.