Treponema pallidum p15, Recombinant (Syphilis)

Catalog No : USB-T8300-17B
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Product name Treponema pallidum p15, Recombinant (Syphilis)
Catalog No USB-T8300-17B
Supplier’s Catalog No T8300-17B
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 30
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% by SDS-PAGE
Form Supplied as a liquid in 20mM Tris, pH 8.0, 8M urea and 10mM BME.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% by SDS-PAGE
Description Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames. p15; Fine Specificity: full length Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.