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Categories: Peptides & Proteins / Proteins

Product name			Treponema pallidum p47, Recombinant (Syphilis)
Catalog No			USB-T8300-19B
Supplier’s Catalog No			T8300-19B
Supplier			US Biologicals
Source antigen			Full length recombinant Treponema pallidum, p47, Beta-galactosidase expressed in E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			160

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% by SDS-PAGE
Form			Supplied as a liquid in 20mM Tris, pH 8.0, 8M urea, 10mM BME.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% by SDS-PAGE
Description			Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.