HIV-1 env gp120, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (HRP)
Catalog No : USB-H6003-07B1
641.40€
0.00€
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| Product name | HIV-1 env gp120, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (HRP) | ||
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| Catalog No | USB-H6003-07B1 | ||
| Supplier’s Catalog No | H6003-07B1 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Baculovirus | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | 4°C | ||
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| Other names | |||
| Grade | Affinity Purified | ||
| Purity | Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced. | ||
| Form | Supplied as a liquid in 50mM Tris, pH 8.0, 1% BSA, 0.01% Thimerosal. Labeled with HRP. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced. | ||
| Description | Peroxidase conjugated rgp120 HIV-1 IIIB (Baculovirus). Molar Ratio: 2-4 peroxidase molecules per molecule of gp120. Specificity: Peroxidase conjugated rgp120 IIIB binds to murine monoclonal antibodies of defined epitope specificity and human monoclonal/polyclonal antibodies in ELISA and Western ELISA. Biological Activity: Peroxidase conjugated rgp120 IIIB binds to immobilized human T-cell receptor CD4 in ELISA and Western ELISA. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1:1000 range depending on the concentration of CD4 in the test system. Binding to murine and human monoclonal/polyclonal antibodies may be performed at higher dilutions. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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