HIV-1 env gp120, Oligomeric, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1)

Catalog No : USB-H6003-07D
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Product name HIV-1 env gp120, Oligomeric, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1)
Catalog No USB-H6003-07D
Supplier’s Catalog No H6003-07D
Supplier US Biologicals
Source antigen Recombinant, Baculovirus
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -70°C
Other names
Grade Affinity Purified
Purity Purified by immunoaffinity chromatography to ≥98% purity as determined by SDS-PAGE, non-reduced and reduced.
Form Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified by immunoaffinity chromatography to ≥98% purity as determined by SDS-PAGE, non-reduced and reduced.
Description Full length HIV-1 IIIB envelope gp120 cloned genes expressing oligomeric glycoprotein produced in the Baculovirus Expression System. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Blot. Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Applications require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 10-100ng of immobilized protein under standard ELISA conditions. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months after receipt.