Imugex

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Categories: Peptides & Proteins / Proteins

Product name			HIV-1 env gp120, Strain MN, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Catalog No			USB-H6003-07F2
Supplier’s Catalog No			H6003-07F2
Supplier			US Biologicals
Source antigen			Recombinant
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			4°C
Other names
Grade			Affinity Purified
Purity			Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Form			Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Labeled with Biotin.
Reactivity life			6 months
Note			For reserch purpose only
Purity			Purified by immunoaffinity chromatography to ≥ 95% purity as determined by SDS-PAGE, reduced.
Description			Coupling Ratio: ~10-20 moles of biotin per mole gp120. Specificity: Biotin gp120 MN binds strepavidin(AP) and streptavidin(HRP) in ELISA and Western Blot assays. Biological Activity: Binds to solid phase CD4 as determined by capture ELISA using strepavidin(HRP) as a secondary reagent. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilution: ELISA: 1:2000-1:10,000 depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and 0.1-1.0ng of biotin-gp120. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.