Imugex

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Categories: Peptides & Proteins / Proteins

Product name			HIV-1 env gp120, Strain MN, Recombinant (Human Immunodeficiency Virus-1) (FITC)
Catalog No			USB-H6003-07F3
Supplier’s Catalog No			H6003-07F3
Supplier			US Biologicals
Source antigen			Baculovirus derived recombinant gp120 from HIV-1 MN strain
Reactivity			This FITC-conjugated gp120 binds to murine monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.
Cross reactivity
Applications
Molecular weight

Storage			-70°C
Other names
Grade			Affinity Purified
Purity			Purified by gel exclusion chromatography and UF concentrated.
Form			Supplied as a liquid in 0.1M NaPO4, 50mM sodium chloride, pH 7.0. No stabilizers or preservatives added. Labeled with FITC.
Reactivity life			6 months
Note			For reserch purpose only
Purity			Purified by gel exclusion chromatography and UF concentrated.
Description			Fluorochrome/Protein Ratio: 2-4 Biological Activity: This FITC-conjugated gp120 binds to CD4 bearing cells in FACS. Applications: Suitable for use in Direct Immunoflouorescent staining, FACS, Immunohistochemistry and Dot Blot. Other applications not tested. Recommended Dilutions: Direct Immunofluorescence: 1:50 Dot Blot: ≥1:100 using purified anti-gp120 monoclonal or polyclonal antibodies. Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable at -70°C for 6 months after receipt. Dilute required amount only prior to immediate use. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.