HIV-1 p66, Strain IIIB, Recombinant (Reverse Transcriptase, Human Immunodeficiency Virus-1)
Catalog No : USB-H6003-15H
649.44€
0.00€
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| Product name | HIV-1 p66, Strain IIIB, Recombinant (Reverse Transcriptase, Human Immunodeficiency Virus-1) | ||
|---|---|---|---|
| Catalog No | USB-H6003-15H | ||
| Supplier’s Catalog No | H6003-15H | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Affinity Purified | ||
| Purity | Purified by ion-affinity chromatography to ≥ 90% purity as determined by SDS-PAGE, reduced. | ||
| Form | Supplied as a liquid in 50mM Tris, pH 8.0, 10% glycerol, 1mM DTT, 0.2M sodium chloride. No stabilizers or preservatives added. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified by ion-affinity chromatography to ≥ 90% purity as determined by SDS-PAGE, reduced. | ||
| Description | Full length Recombinant heterodimeric HIV-1 IIIB p66 (RT) produced in E.coli expression system. Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA. Biological Activity: 0.1 unit of purified RT incorporates a minimum of 5 picomoles of dTTP per minute under standard RT assay conditions. Applications: Suitable for use in Dot Blot, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western Blot require 1-10ng protein depending on the nature and affinity of the test antibody. Solid phase antigen in 50-100ng amounts shows reactivity with human serum antibodies in Dot Blot assays. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing. Aliquots are stable 6 months after receipt.at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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