Imugex

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Categories: Peptides & Proteins / Proteins

Product name			HIV-1 gp120, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1)
Catalog No			USB-H6003-34E
Supplier’s Catalog No			H6003-34E
Supplier			US Biologicals
Source antigen			CHO cells
Reactivity			This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western ELISA.
Cross reactivity
Applications
Molecular weight			120

Storage			-70°C
Other names
Grade			Affinity Purified
Purity			Purified by immunoaffinity chromatography to ≥95% purity as determined by SDS-PAGE, reduced.
Form			Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
Reactivity life			6 months
Note			For reserch purpose only
Purity			Purified by immunoaffinity chromatography to ≥95% purity as determined by SDS-PAGE, reduced.
Description			Full length recombinant protein corresponding to HIV-1 IIIB gp120 glycoprotein produced in the CHO Expression System. Biological Activity: This protein binds to human T-cell receptor CD4 as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells. Applications: Suitable for use in ELISA and Western ELISA. Also suitable for use in CD4 binding and T-cell activation studies. Other applications have not been tested. Recommended Dilutions: ELISA: 10-100ng Western Blot: 10-100ng Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.