HIV-1 nef, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)

Catalog No : USB-H6004-18B
705.77€
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Product name HIV-1 nef, Strain IIIB, Recombinant (Human Immunodeficiency Virus-1) (Biotin)
Catalog No USB-H6004-18B
Supplier’s Catalog No H6004-18B
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight
Storage 4°C
Other names
Grade Affinity Purified
Purity Purified by glutathione affinity chromatography and the purified protein is thrombin-cleaved to produce nef of ≥95% purity, as determined by SDS-PAGE, reduced.
Form Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Reactivity life 6 months
Note For reserch purpose only
Purity Purified by glutathione affinity chromatography and the purified protein is thrombin-cleaved to produce nef of ≥95% purity, as determined by SDS-PAGE, reduced.
Description Coupling Ratio: ~5-10 moles of biotin per mole nef. Biological Activity: Binds to solid phase murine anti-nef monoclonal antibodies as determined by capture ELISA using strepavidin-HRP as a secondary reagent. Applications: Suitable for use in T-Cell Research, ELISA and Western ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Capture ELISA: 1-10ng Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.