HIV-1 tat, Strain MN, Recombinant (Human Immunodeficiency Virus-1)
Catalog No : USB-H6007-11
1036.81€
0.00€
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| Product name | HIV-1 tat, Strain MN, Recombinant (Human Immunodeficiency Virus-1) | ||
|---|---|---|---|
| Catalog No | USB-H6007-11 | ||
| Supplier’s Catalog No | H6007-11 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥95% (SDS-PAGE and HPLC) | ||
| Form | Supplied as a liquid in 0.2M KCl, 5mM glutathione. No stabilizers or preservatives added. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥95% (SDS-PAGE and HPLC) | ||
| Description | Full length (100 amino acids) Recombinant HIV-1 MN tat produced in the E. coli Expression System. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies in ELISA and Western ELISA. Biological Activity: The biological specificity of this protein was determined by LTR-Beta galactosidase induction in MAGI and MAGI-CCR5 cells. Tat in 1-10ug/ml range induced Beta- galactosidase activity 30-fold over un-treated cells. Endotoxin: Less than 0.01EU/mg of protein as determined by BioWhittaker Kinetic QCL Kit. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute tat stock solution in saline-phosphate buffer (150mM NaCl, 50mM sodiumphosphate, pH 6.5) immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity. Transcriptional activation assays with tat are performed in 1-10µg/ml range. ELISA and Western ELISA require tat in 10-100ng protein range. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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