Hepatitis C Virus, Combined, Recombinant Nucleocapsid, NS3, NS4, and NS5) (soluble)
Catalog No : USB-H1919-85E
747.15€
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| Product name | Hepatitis C Virus, Combined, Recombinant Nucleocapsid, NS3, NS4, and NS5) (soluble) | ||
|---|---|---|---|
| Catalog No | USB-H1919-85E | ||
| Supplier’s Catalog No | H1919-85E | ||
| Supplier | US Biologicals | ||
| Source antigen | E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Affinity Purified | ||
| Purity | Purified by affinity chromatography. ≥95% by 10% PAGE (coomassie staining) | ||
| Form | Supplied as a liquid 50mM sodium phophate, pH 8.5, 2.4mM EDTA, 5mM DTT, 0.1% SDS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | Purified by affinity chromatography. ≥95% by 10% PAGE (coomassie staining) | ||
| Description | Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then constructed into one recombinant antigen. The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions. Immunoreactivity: Immunoreactive with sera of HCV-infected individuals, including sera with antibodies to NS3 secondary structure domains. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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