Bcl 2 (BCL2, Bcl-2, B Cell CLL/lymphoma 2) (Control Peptide)

Catalog No : USB-B0807-02E-P
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Product name Bcl 2 (BCL2, Bcl-2, B Cell CLL/lymphoma 2) (Control Peptide)
Catalog No USB-B0807-02E-P
Supplier’s Catalog No B0807-02E-P
Supplier US Biologicals
Source antigen Synthetic
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity Highly Purified
Form Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.
Reactivity life 12 months
Note For reserch purpose only
Purity Highly Purified
Description AGRTGYDNREIVMKYC located at the N Terminus of the protein sequence. Control peptide corresponding to polyclonal antibody, Catalog#B0807-02E, goat x human. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody B0807-02E): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.