Adenovirus, Strain Adenoid 6

Catalog No : USB-A0876-35A
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Product name Adenovirus, Strain Adenoid 6
Catalog No USB-A0876-35A
Supplier’s Catalog No A0876-35A
Supplier US Biologicals
Source antigen MRC-S Cells
Reactivity
Cross reactivity
Applications
Molecular weight 30
Storage -70°C
Other names
Grade Lysate
Purity Lysate
Form Supplied as a liquid in MEM. No preservative added.
Reactivity life 12 months
Note For reserch purpose only
Purity Lysate
Description Adenoviridae family. A non enveloped, icosahedral virion, 75-80 nm diameter, double-stranded, linear DNA genome. Varies in clinical manifestation and severity. Symptoms include fever, rhinitis, pharyngitis, cough and conjunctivitis. Contains high concentration of virus and viral components as well as some cellular material. Source: Adenovirus, Strain Adenoid 6, expressed in MRC-5 cells Inactivation: Gamma radiation. Purification: Optimally infected cells are disrupted by sonication and then subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated by cross flow ultrafiltration with a 30,000 molecular weight cut-off. Applications: Suitable for use in ELISA with polystyrene and latex solid phases for detection of both IgG and IgM. Other applications not tested. Recommended Dilution: The antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. This preparation may be further purified to meet the needs of a particular assay format. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.