Rubella, E1, Recombinant (German Measles)
Catalog No : USB-R9700-05K
1427.63€
0.00€
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| Product name | Rubella, E1, Recombinant (German Measles) | ||
|---|---|---|---|
| Catalog No | USB-R9700-05K | ||
| Supplier’s Catalog No | R9700-05K | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 32 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (10% PAGE, coomassie staining). | ||
| Form | Supplied as a liquid in 20mM imidazole, 8M urea, 0.3M sodium chloride. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (10% PAGE, coomassie staining). | ||
| Description | Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins, and C capsid protein. E1 envelope glycoprotein is a class II viral fusion protein. Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and endocytosis, acidification of the endosome induces dissociation of E1/E2 heterodimer and trimerization of the E1 subunits. This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in the cytoplasm after cell and viral membrane fusion. The E1 cytoplasmic tail modulates virus release, and the tyrosines residues are critical for this function. Contains a mosaic of the Rubella virus E1 regions. Does not contain a fusion partner. Immunoreactive with Rubella virus positive sera. Source: E. coli Molecular Weight: ~32kD Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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