Rubella, E2, Recombinant (German Measles)

Catalog No : USB-R9700-06D
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Product name Rubella, E2, Recombinant (German Measles)
Catalog No USB-R9700-06D
Supplier’s Catalog No R9700-06D
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 33
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (10% PAGE, coomassie staining).
Form Supplied as a liquid in 20mM imidazole, 8M urea, 0.3M sodium chloride.
Reactivity life 6 months
Note For reserch purpose only
Purity ~95% (10% PAGE, coomassie staining).
Description Rubella virus is the only member of the Rubrivirus genus of the Togavirus family. Unlike most Togaviruses it is NOT arthropod borne, but is acquired via the respiratory route. It is an enveloped (toga=cloak), non-segmented, positive sense, RNA virus and replicates in the cytoplasm. It consists of 3 structural proteins; E1,E2 membrane bound glycoproteins, and C capsid protein. The the two envelope glycoproteins E2 and E1 are found as a heterodimeric spike complex embedded in the lipid envelope. E2 envelope glycoprotein is responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. Contains the Rubella virus E2 region. Does not contain a fusion partner. Immunoreactive with Rubella virus positive sera. Source: E. coli Molecular Weight: ~33kD Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.