Treponema pallidum p41, Recombinant (Syphillis)

Catalog No : USB-T8300-05B
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Product name Treponema pallidum p41, Recombinant (Syphillis)
Catalog No USB-T8300-05B
Supplier’s Catalog No T8300-05B
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 37
Storage -20°C
Other names
Grade Highly Purified
Purity ~90% (10% PAGE, coomassie staining). GS-4B Sepharose-Affinity Purification.
Form Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 60mM sodium chloride, 50% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% (10% PAGE, coomassie staining). GS-4B Sepharose-Affinity Purification.
Description Contains a mosaic of the immunodominant regions of Treponema pallidum p41. Not full-length p41. Contains GST Fusion partner. Molecular weight is 37kD. Immunoreactive with T. pallidum positive sera. Treponema pallidum is the causative agent of syphilis. It is a spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella. Mechanisms of T. pallidum pathogenesis are poorly understood. No known virulence factors have been identified, and the outer membrane is mostly lipid with a paucity of proteins. Consequently, existing diagnostic tests for syphilus are suboptimal, and no vaccine against T. pallidum is available. TIGR sequenced the genome of T. pallidum subsp. pallidum (strain Nichols) by the whole genome random sequencing method. The T. pallidum genome is a circular chromosome of 1,138,006 base pairs containing a total of 1041 predicted open reading frames. Source: E. coli Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.