Hepatitis A Virus, pHM175 (HAV)

Catalog No : USB-H1900-01B
397.71€
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Product name Hepatitis A Virus, pHM175 (HAV)
Catalog No USB-H1900-01B
Supplier’s Catalog No H1900-01B
Supplier US Biologicals
Source antigen FRhK-4 cells
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -70°C
Other names
Grade Purified
Purity ~60% viral protein.
Form Supplied as a liquid in 0.01M Tris, pH 8.0-8.50, 0.15M sodium chloride, 1mM EDTA, 0.085% sodium azide.
Reactivity life 12 months
Note For reserch purpose only
Purity ~60% viral protein.
Description Hepatitis A Virus (HAV) is the cause of infectious hepatitis transmitted via the fecal-oral route. The clinical course of HAV infection can range from a mild anicteric illness, to severe prolonged icteric hepatitis. Hepatitis A Virus (HAV) is classified with the enterovirus group of RNA virus causing Type A viral hepatitis. Hepatitis A is usually a mild illness characterized by sudden on-set fever, malaise, nausea, anorexia, and abdominal discomfort followed in several days by jaundice. The Centers for Disease Control estimates that 143,000 cases of acute HAV infection occur each year in the U.S.A. and worldwide estimates of HAV infection exceeds 1.4 million cases. The diagnosis of acute or past infections with Hepatitis A virus can be provided by the evaluation of the patients IgM and total immunoglobulin antibody to HAV. Anti-HAV IgM is almost always present in the patient serum at the appearance of symptoms and may be present for several months after acute illness. The total immunoglobulin antibody level to HAV is positive in acute Hepatitis A infection and remains positive indefinitely. Source: Hepatitis A virus propagated in FRhK-4 cells infected with the HAV virus strain pHM175. Infected cells are harvested and the virus is partially purified prior to the inactivation. Inactivation: Inactived with Fomalin Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: Capture Western Blot: Detects a band at ~33kD and a band in the 24-27kD region. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.