Dengue Virus, Type 2 (DEN2)

Catalog No : USB-D2800-13B
1059.80€
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Product name Dengue Virus, Type 2 (DEN2)
Catalog No USB-D2800-13B
Supplier’s Catalog No D2800-13B
Supplier US Biologicals
Source antigen Native
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -70°C
Other names
Grade Inactivated Pathogen
Purity
Form Supplied as a liquid in Medium 199. Preservative free. Inactivated.
Reactivity life 12 months
Note For reserch purpose only
Purity
Description Dengue infection is a major cause of morbidity in tropical and subtropical regions. It is a mosquito borne viral infection that may be asymptomatic or may cause undifferentiated fever, dengue fever, dengue haemorrhagic fever or dengue shock syndrome. The Dengue virus group consists of four closely related serotypes and belongs to the family Flaviviridae. Currently there is no vaccine or effective antiviral drugs to treat the infection. Source: Dengue virus type 2 strain 16681 cultured in Vero cells. Preparation: Dengue virus particles are concentrated from tissue culture supernatant by precipitation and ultracentrifugation. Antigen is purified by sucrose density gradient centrifugation. Virus particles are separated from the sucrose containing buffer by ultracentrifugation before resuspension in Medium 199. The antigen preparation is inactivated by room temperature incubation with formaldehyde. The formaldehyde is neutralized by the addition of sodium bisulphite. This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.