Hepatitis C Virus NS3, aa1225-1456, Recombinant (HCV)

Catalog No : USB-H1920-23E8
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Product name Hepatitis C Virus NS3, aa1225-1456, Recombinant (HCV)
Catalog No USB-H1920-23E8
Supplier’s Catalog No H1920-23E8
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 27.9
Storage -20°C
Other names
Grade Highly Purified
Purity ~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.
Form Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.
Description HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E. coli. Source: Recombinant corresponding to aa1225-1456 of human HCV NS3 6x His tagged expressed in E. coli. AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.