HCV1a (D168V), Recombinant (Hepatitis C virus genotype 1a)

Catalog No : USB-171869
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Product name HCV1a (D168V), Recombinant (Hepatitis C virus genotype 1a)
Catalog No USB-171869
Supplier’s Catalog No 171869
Supplier US Biologicals
Source antigen Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight 22.4
Storage -70°C
Other names
Grade Purified
Purity ~53% (SDS-PAGE)
Form Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween 20, 250mM imidazole, 20% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~53% (SDS-PAGE)
Description Fusion protein corresponding to the serine protease NS3 (a.a.3-181) and cofactor NS4A (a.a.21-32) from Hepatitis C virus genotype 1a (GenBank Accession No. NC_004102) with Asp-to-Val mutation on a.a.168, and N-terminal FLAG-His tag, MW=22.4kD, expressed in an E. coli expression system. Source: Recombinant protein corresponding to aa3-181 from HCV1a at the N-terminus, with Asp-to-Val mutation on aa 168, an N-terminal FLAG-His-Tag, and a 4aa linker, expressed in E.coli. Molecular Weight: ~22.4kD Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. AA Sequence: MDYKDDDDKHHHHHHGCVVIVGRIVLSGSGSITAYAQQTRGLLGCIITSLTGRDKNQVEGEVQIVSTATQTFLATCINGVCWTVYHGAGTRTIASPKGPVIQMYTNVDQDLVGWPAPQGSRSLTPCTCGSSDLYLVTRHADVIPVRRRGDSRGSLLSPRPISYLKGSSGGPLLCPAGHAVGLFRAAVCTRGVAKAVVFIPVENLETTMRS Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap