Hemagglutinin Influenza A Virus H1N1 New Caledonia 20/99, Recombinant

Catalog No : USB-208949
417.25€
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Product name Hemagglutinin Influenza A Virus H1N1 New Caledonia 20/99, Recombinant
Catalog No USB-208949
Supplier’s Catalog No 208949
Supplier US Biologicals
Source antigen Recombinant, Baculovirus Insect cells
Reactivity
Cross reactivity
Applications
Molecular weight 57.5
Storage -20°C
Other names
Grade Purified
Purity ~90% (SDS-PAGE, HA1 and HA2 bands are observed using SDS-PAGE under reducing conditions)
Form Supplied as a liquid in 10mM sodium phosphate, pH 7.2, 150mM sodium chloride, 0.005% tween 20.
Reactivity life 12 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE, HA1 and HA2 bands are observed using SDS-PAGE under reducing conditions)
Description H1N1 is a subtype specie of Influenza A virus. H1N1 Influenza Virus has mutated into various strains such as the Spanish Flustrain, mild humanflu strains, endemic pigstrains, and various strains found in birds. The Influenza A Virus is a globular particle about 100nm in diameter, sheathed in a lipid bilayer derived from the plasma membrane of its host. Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin ("H") and some 100 molecules of neuraminidase ("N"). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some "non-structural" protein molecules of uncertain function. H1N1 New Caledonia shows 90% similarity to the A/PR/8/34 amino acid sequence. Source: Recombinant protein corresponding to full-Length H1N1 A/New Caledonia/20/99, glycosylated with N-linked sugars, expressed in baculovirus insect cells. Molecular Weight: ~57.5kD Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1ug/well Western Blot: 0.1-1ug/strip Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.