Hepatitis B Surface Antigen, preS1, Recombinant (HBsAg, HBV)

Catalog No : USB-208967
473.58€
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Product name Hepatitis B Surface Antigen, preS1, Recombinant (HBsAg, HBV)
Catalog No USB-208967
Supplier’s Catalog No 208967
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 12.6
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (10% PAGE, coomassie staining)
Form Supplied as a lyophilized powder in 20mM PB, pH 7.4, 50mM sodium chloride. Reconstitute in sterile ddH2O or aqueous buffer, 0.1% BSA.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (10% PAGE, coomassie staining)
Description Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119aa region called preS1. Source: Recombinant protein corresponding to Hepatitis B Surface Antigen preS1, a single non-glycosylated polypeptide chain containing 119aa, expressed in E. coli. Molecular Weight: ~12.6kD AA Sequence: MGGWSSKPRQGMGTNLSVPNPLGFFPDHQLDPAFG ANSNNPDWDFNPNKDHWPEAHQVGAGAFGPGFTP PHGGLLGWSPQAQGILTTVPVAPPPASTNRQSGRQP TPISPPLRDSHPQA Applications: Suitable for use in ELISA, Immunochromatography (capture and conjugate) and preparing monoclonal or polyclonal antibodies for HBsAg-preS1. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.