Hepatitis B Virus, Core Delta, Recombinant, aa1-144 (HBV)
Catalog No : USB-208970
531.05€
0.00€
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| Product name | Hepatitis B Virus, Core Delta, Recombinant, aa1-144 (HBV) | ||
|---|---|---|---|
| Catalog No | USB-208970 | ||
| Supplier’s Catalog No | 208970 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 16 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (10% PAGE, coomassie staining) | ||
| Form | Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1mM EDTA, 50% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (10% PAGE, coomassie staining) | ||
| Description | Hepatitis B is one of a few known non-retroviral viruses which employ reverse transcription as a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA. Source: Recombinant protein corresponding to the HBV core delta, ayw immunodominant region, aa1-144, expressed in E. coli. Molecular Weight: ~16kD Specificity: Immunoreactive with sera of HBV-infected individuals. Applications: Suitable for use in ELISA, Western Blot and as an antigen for detection of HBV with minimal specificity problems. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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