Hepatitis C Virus, NS4 a+b, Recombinant, aa1658-1863 (HCV) (Biotin)

Catalog No : USB-208976
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Product name Hepatitis C Virus, NS4 a+b, Recombinant, aa1658-1863 (HCV) (Biotin)
Catalog No USB-208976
Supplier’s Catalog No 208976
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 19
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (10% PAGE, coomassie staining)
Form Supplied as a liquid in 20mM Tris-Hcl, pH 8.0, 8M urea, 10mM B-ME. No preservative added. Labeled with Biotin.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (10% PAGE, coomassie staining)
Description HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with ~one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes (1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6). Source: Recombinant protein corresponding to the HCV NS4 immunodominant regions, aa1658-1863, fused with b-galactosidase (~114kD) at N-terminus, expressed in E. coli. Molecular Weight: ~19kD Specificity: Immunoreactive with sera of HCV-infected individuals. Applications: Suitable for use as an antigen in ELISA, Western Blot and is an excellent antigen for detection of HCV with minimal specificity problems. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.