Pertussis Toxin (Islet Activating Protein, B. pertussis)
Catalog No : USB-209138
758.64€
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| Product name | Pertussis Toxin (Islet Activating Protein, B. pertussis) | ||
|---|---|---|---|
| Catalog No | USB-209138 | ||
| Supplier’s Catalog No | 209138 | ||
| Supplier | US Biologicals | ||
| Source antigen | B. pertussis | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified (~95%) | ||
| Form | Supplied as a liquid in 0.05M Tris, 0.01M glycine, 0.5M NaCI, pH 7.5, 50% glycerol. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified (~95%) | ||
| Description | Pertussis toxin (PT) is a protein-based AB5-type exotoxin produced by the bacterium Bordetella pertussis, which causes whooping cough. PT is involved in the colonization of the respiratory tract and the establishment of infection. PT may have a therapeutic role in treating a number of common human ailments, including hypertension, viral inhibition, and autoimmune inhibition. A large group of bacterial exotoxins are referred to as "A/B toxins", in essence because they are formed from two subunits. The "A" subunit possesses enzyme activity, and is transferred to the host cell following a conformational change in the membrane-bound transport "B" subunit. Pertussis toxin is an exotoxin with six subunits (named S1 through S5-each complex contains two copies of S4). The subunits are arranged in A-B structure: the A component is enzymatically active and is formed from the S1 subunit, while the B component is the receptor-binding portion and is made up of subunits S2-S5. The subunits are encoded by ptx genes encoded on a large PT operon that also includes additional genes that encode Ptl proteins. Together, these proteins form the PT secretion complex. B. pertussis expresses two forms of a rough-type lipooligosaccharide (LOS), which have been referred to as LOS A and LOS B. The two forms of LOS can be resolved as two distinct molecular weight species in silver stained sodium dodecyl sulfate-polyacrylamide gels of protease-treated B. pertussis cell lysates. The difference between the two forms of LOS has been shown to be due to three additional N-acetyl amino sugars in the core oligosaccharide moiety of LOS A that are not present in LOS B. The structure and biological activity of B. pertussis LOS are similar to those of LOSs of other gram negative bacteria that contain lipid A and a core oligosaccharide moiety containing 2-keto-3-deoxyoctonate, exhibiting lethal toxicity in mice, pyrogenicity in rabbits, and mitogenicity in cell cultures. Toxoid refers to the inactivated version of the active toxin. It is typically produced by formaldehyde inactivation of the active protein. Source: Pertussis toxin, from B. pertussis Biological Activity: The lowest concentration of toxin at which a positive response (clustered growth pattern) was obtained was 0.33ng/ml in CHO cells. Specific Activity: The adenylate cyclase activity of this lot is 4.5 pmole/min/ug in the presence of 1uM calmodulin. Applications: Suitable for use in ELISA, Western Blot, Antibody Titration or as control protein for Adjuvant. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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