POLV1-VP1, Recombinant, His-Tag (Sabin, Poliomyelitis Virus 1 Viral Protein 1)
Catalog No : USB-209148
644.85€
0.00€
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| Product name | POLV1-VP1, Recombinant, His-Tag (Sabin, Poliomyelitis Virus 1 Viral Protein 1) | ||
|---|---|---|---|
| Catalog No | USB-209148 | ||
| Supplier’s Catalog No | 209148 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 32 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified (~95%) | ||
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.25M sodium chloride, 5mM beta- mercaptoethanol, 0.25M imadazole, 8M urea. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified (~95%) | ||
| Description | Poliomyelitis, often called polio or infantile paralysis, is an acute viral infectious disease spread from person to person, primarily via the fecal-oral route. Although around 90% of polio infections cause no symptoms at all, affected individuals can exhibit a range of symptoms if the virus enters the blood stream. Spinal polio is the most common form, characterized by asymmetric paralysis that most often involves the legs. Bulbar polio leads to weakness of muscles innervated by cranial nerves. Bulbospinal polio is a combination of bulbar and spinal paralysis. The term poliomyelitis is used to identify the disease caused by any of the three serotypes of poliovirus. Type 1 (Brunhilde): often with severe symptoms Type 2 (Lansing): with milder symptoms Type 3 (Leon): rare, but with severe symptoms. Two basic patterns of polio infection are described: a minor illness which does not involve the central nervous system (CNS), sometimes called abortive poliomyelitis, and a major illness involving the CNS, which may be paralytic or non-paralytic. A laboratory diagnosis is usually made based on recovery of poliovirus from a stool sample or a swab of the pharynx. Antibodies to poliovirus can be diagnostic, and are generally detected in the blood of infected patients early in the course of infection. Poliovirus is a human enterovirus and member of the family of Picornaviridae. It composed of an ss-positive sense RNA genome (~7500nt) and a protein capsid. Because of its short genome and its simple composition-only RNA and a non-enveloped icosahedral protein coat that encapsulates it-poliovirus is widely regarded as the simplest significant virus. Poliovirus mRNA is translated as one long polypeptide. This polypeptide is then auto-cleaved by internal proteases into ~10 individual viral proteins, including: 3Dpol, an RNA dependent RNA polymerase; 2Apro and 3Cpro/3CDpro, proteases which cleave the viral polypeptide; VPg (3B), a small protein that binds viral RNA and is necessary for synthesis of viral positive and negative strand RNA; 2BC, 2B, 2C, 3AB, 3A, 3B proteins which comprise the protein complex needed for virus replication; VP0, VP1, VP2, VP3, VP4 proteins of the viral capsid. Capsid proteins VP1, VP2, VP3 and VP4 form a closed capsid enclosing the viral positive strand RNA genome. VP4 lies on the inner surface of the protein shell formed by VP1, VP2 and VP3. Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, VP2, and VP3. The capsid interacts with human PVR at this site to provide virion attachment to target cell. Poliovirus is structurally similar to other human enteroviruses (coxsackieviruses, echoviruses, and rhinoviruses), which also use immunoglobulin-like molecules to recognize and enter host cells. There are three serotypes of poliovirus, PV1, PV2, and PV3; each with a slightly different capsid protein. Capsid proteins define cellular receptor specificity and virus antigenicity. PV1 is the most common form encountered in nature, however all three forms are extremely infectious. Wild polioviruses can be found in two continents. As of 2012, PV1 is highly localized to regions in Pakistan and Afghanistan in Asia, and Nigeria, Niger and Chad in Africa. Wild poliovirus type 2 has probably been eradicated; it was last detected in October 1999 in Uttar Pradesh, India. Wild PV3 is found in parts of only two countries, Nigeria and Pakistan. Specific strains of each serotype are used to prepare vaccines against polio. Inactive polio vaccine (IPV) is prepared by formalin inactivation of three wild, virulent reference strains, Mahoney or Brunenders (PV1), MEF-1/Lansing (PV2), and Saukett/Leon (PV3). Oral polio vaccine (OPV) contains live attenuated (weakened) strains of the three serotypes of poliovirus. Passaging the virus strains in monkey kidney epithelial cells introduces mutations in the viral IRES, and hinders (or attenuates) the ability of the virus to infect nervous tissue. Poliovirus capsid protein VP1 is one of four structural proteins and its antigenic. The N-termini of most EV VP1 proteins contain highly conserved immunogenic regions that are recognized by sera from most EV-infected patients. Poliovirus VP1 has been considered a candidate for recombinant poliovirus subunit vaccine. Source: Recombinant protein corresponding to aa1-301 from Poliovirus VP1 Sabin strain, fused to His-tag, expressed in E. coli. Molecular Weight: ~32kD Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: coat at ~1-5ug/ml and test with appropriate antibodies Western Blot: load ~250-500ng/well and detect with appropriate antibodies Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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